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Fig. 1 | Thyroid Research

Fig. 1

From: Activating transcription factor 3 mediates apoptosis and cell cycle arrest in TP53-mutated anaplastic thyroid cancer cells

Fig. 1

Fluorescent intensity for pCMV6-ATF3-tranfected, mock-transfected, and control 8305 C cell groups using fluorescence microscopy and flow cytometry. (A) 8305 C cells transfected with 6 µg of the pCMV6-ATF3, empty vector (mock), and control group (8305 C cells were cultured under the same conditions, but without transfection agents). Cells were observed using a 20-fold objective lens. (B) Histograms from FACS analysis for 6 µg of pCMV6-ATF3, mock-transfected, and control group. (C) Average fluorescent intensity for pCMV6-ATF3, mock-transfected, and control group. Flow cytometry analysis showed that the average transfection rates for pCMV6-ATF-transfected cells after 48 and 72 h were 69.7% and 86.6%, respectively. **** P ≤ 0.0001

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Thyroid Research

ISSN: 1756-6614